Effect of Brn-3a deficiency on parvalbumin-, calbindin D-28k-, calretinin- and calcitonin gene-related peptide-immunoreactive primary sensory neurons in the trigeminal ganglion

Neuroscience. 2002;113(3):537-46. doi: 10.1016/s0306-4522(02)00182-3.

Abstract

Immunohistochemistry for parvalbumin, calbindin D-28k, calretinin and calcitonin gene-related peptide (CGRP) was performed on the trigeminal ganglion and oro-facial tissues in Brn-3a wildtype and knockout mice at embryonic day 18.5 and postnatal day 0. In wildtype mice, the trigeminal ganglion contained abundant parvalbumin-, calbindin D-28k- and CGRP-immunoreactive neurons while the ganglion was almost devoid of calretinin-immunoreactive neurons. In Brn-3a knockout mice, a 63% decrease of parvalbumin-immunoreactive neurons was detected. In contrast, the absence of Brn-3a dramatically increased the number of calbindin D-28k-immunoreactive (3.5-fold increase) and calretinin-immunoreactive neurons (91-fold increase). The number of CGRP-immunoreactive neurons, however, was not altered by the Brn-3a deficiency. Cell size analysis indicated that loss of Brn-3a increased the proportions of small (<100 microm (2)) parvalbumin-, calbindin D-28k- and CGRP-immunoreactive neurons while it decreased those of large (>200 microm(2)) immunoreactive cells. Calretinin-immunoreactive neurons were either small or medium (100-200 microm (2)) in mutant mice. The oro-facial tissues contained parvalbumin-, calbindin D-28k- and CGRP-immunoreactive fibers, but not calretinin-immunoreactive ones in wildtype mice. In Brn-3a knockout mice, the number of parvalbumin-immunoreactive fibers markedly decreased in the infraorbital nerve and parvalbumin-immunoreactive endings disappeared in the vibrissa. In contrast, the number of calbindin D-28k-immunoreactive fibers increased significantly in the infraorbital and mental nerves. In addition, calbindin D-28k-immunoreactive endings appeared in the vibrissa. As well, some fibers showed calretinin-immunoreactivity in the infraorbital nerve of the mutant. However, no obvious change of CGRP-immunoreactive fibers was observed in the oro-facial region of knockout mice. Taken together, our data suggest that Brn-3a deficiency has effects on the expression of neurochemical substances in the trigeminal ganglion.

MeSH terms

  • Animals
  • Animals, Newborn
  • Calbindin 2
  • Calbindins
  • Calcitonin Gene-Related Peptide / analysis*
  • Calcitonin Gene-Related Peptide / immunology
  • DNA-Binding Proteins / deficiency*
  • DNA-Binding Proteins / genetics
  • Face / innervation
  • Immunohistochemistry
  • Mice
  • Mice, Knockout
  • Nerve Fibers / chemistry
  • Nerve Fibers / metabolism
  • Neurons, Afferent / chemistry
  • Neurons, Afferent / metabolism*
  • Parvalbumins / analysis*
  • Parvalbumins / immunology
  • S100 Calcium Binding Protein G / analysis*
  • S100 Calcium Binding Protein G / immunology
  • Transcription Factor Brn-3
  • Transcription Factor Brn-3A
  • Transcription Factors / deficiency*
  • Transcription Factors / genetics
  • Trigeminal Ganglion / chemistry
  • Trigeminal Ganglion / embryology
  • Trigeminal Ganglion / metabolism*
  • Vibrissae / innervation

Substances

  • Calb2 protein, mouse
  • Calbindin 2
  • Calbindins
  • DNA-Binding Proteins
  • Parvalbumins
  • Pou4f1 protein, mouse
  • S100 Calcium Binding Protein G
  • Transcription Factor Brn-3
  • Transcription Factor Brn-3A
  • Transcription Factors
  • Calcitonin Gene-Related Peptide