Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Bioorg Med Chem. 2002 Oct;10(10):3313-8. doi: 10.1016/s0968-0896(02)00204-3.

Abstract

The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Boron Compounds / pharmacokinetics
  • Cell Compartmentation
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Fluorescent Dyes / pharmacokinetics*
  • Humans
  • Imidazoles / pharmacokinetics*
  • Microscopy, Confocal
  • Nylons / pharmacokinetics*
  • Pyrroles / pharmacokinetics*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / ultrastructure
  • Tumor Cells, Cultured

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Imidazoles
  • Nylons
  • Pyrroles
  • N-methylpyrrole
  • 1-methylimidazole