BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling

Cell. 2002 Jul 26;110(2):213-22. doi: 10.1016/s0092-8674(02)00812-7.

Abstract

Brassinosteroids regulate plant growth and development through a protein complex that includes the leucine-rich repeat receptor-like protein kinase (LRR-RLK) brassinosteroid-insensitive 1 (BRI1). Activation tagging was used to identify a dominant genetic suppressor of bri1, bak1-1D (bri1-associated receptor kinase 1-1Dominant), which encodes an LRR-RLK, distinct from BRI1. Overexpression of BAK1 results in elongated organ phenotypes, while a null allele of BAK1 displays a semidwarfed phenotype and has reduced sensitivity to brassinosteroids (BRs). BAK1 is a serine/threonine protein kinase, and BRI1 and BAK1 interact in vitro and in vivo. Expression of a dominant-negative mutant allele of BAK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles. These results indicate BAK1 is a component of BR signaling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Arabidopsis
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Brassinosteroids
  • Cholestanols / pharmacology
  • Enzyme Activation
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Hydroxysteroids / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Phenotype
  • Phosphorylation
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Signal Transduction / physiology*
  • Steroids, Heterocyclic / pharmacology
  • Tissue Distribution

Substances

  • Arabidopsis Proteins
  • Brassinosteroids
  • Cholestanols
  • Hydroxysteroids
  • Membrane Proteins
  • Repressor Proteins
  • Steroids, Heterocyclic
  • Protein Kinases
  • BAK1 protein, Arabidopsis
  • BRI1 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases
  • brassinolide