AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis

Biochim Biophys Acta. 2002 Aug 19;1577(1):126-32. doi: 10.1016/s0167-4781(02)00411-6.


AMY-1 has been identified by us as a c-Myc-binding protein and was found to stimulate c-Myc transcription activity. AMY-1 was also found to be associated with AKAP84/149 in the mitochondria in somatic cells and sperm, suggesting that it plays a role in spermatogenesis. To access the molecular function of AMY-1, a two-hybrid screening of cDNAs encoding AMY-1-binding proteins was carried out with AMY-1 as a bait using a human testis cDNA library, and a clone encoding a novel protein, AMAP-1, was obtained. The amap-1 gene was mapped at human chromosome 17q21. AMY-1 was found to bind to and be colocalized with AMAP-1 in human 293T and HeLa cells. AMAP-1 was found to be specifically expressed in the testis and expressed post-meiotically in the testis, as was AMY-1. These results suggest that both AMAP-1 and AMY-1 play roles in spermatogenesis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics*
  • Chromosomes, Human, Pair 17
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Kinetics
  • Male
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis
  • Salivary alpha-Amylases
  • Spermatogenesis*
  • Testis / growth & development
  • Testis / metabolism*
  • Testis / physiology
  • Tissue Distribution
  • Transcription Factors / metabolism*
  • Transcription, Genetic


  • Carrier Proteins
  • DNA-Binding Proteins
  • MYCBP protein, human
  • MYCBPAP protein, human
  • RNA, Messenger
  • Transcription Factors
  • Amy1 protein, mouse
  • Salivary alpha-Amylases

Associated data

  • GENBANK/AB083068