Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin

J Biol Chem. 2002 Oct 11;277(41):38596-606. doi: 10.1074/jbc.M205005200. Epub 2002 Jul 31.


Tyrosine phosphorylation evokes functional changes in a variety of ion channels. Modulation of the actin cytoskeleton also affects the function of some channels. Little is known about how these avenues of ion channel regulation may interact. We report that the potassium channel Kv1.2 associates with the actin-binding protein cortactin and that the binding is modulated by tyrosine phosphorylation. Immunocytochemical and biochemical analyses show that Kv1.2 and cortactin co-localize to the cortical actin cytoskeleton at the leading edges of the cell. Binding assays using purified recombinant proteins reveal a 19-amino acid span within the carboxyl terminus of Kv1.2 that is necessary for direct cortactin binding. Phosphorylation of specific tyrosines within the C terminus of Kv1.2 attenuates that binding. In HEK293 cells, activation of the M1 muscarinic acetylcholine receptor evokes tyrosine phosphorylation-dependent suppression of Kv1.2 ionic current. We show that M1 receptor activation also reduces the interaction of cortactin with Kv1.2 and that mutant Kv1.2 channels deficient for cortactin binding exhibit strongly attenuated ionic current. These results demonstrate a dynamic, phosphorylation-dependent interaction between Kv1.2 and the actin cytoskeleton-binding protein cortactin and suggest a role for that interaction in the regulation of Kv1.2 ionic current.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism
  • Animals
  • Brain Chemistry
  • Cell Fractionation
  • Cell Line
  • Cortactin
  • Cytoskeleton / metabolism
  • Humans
  • Immunohistochemistry
  • Kv1.2 Potassium Channel
  • Microfilament Proteins / metabolism*
  • Mutagenesis, Site-Directed
  • Oocytes / physiology
  • Patch-Clamp Techniques
  • Phosphorylation
  • Potassium Channels / genetics
  • Potassium Channels / metabolism*
  • Potassium Channels, Voltage-Gated*
  • Protein Binding
  • Protein Structure, Tertiary
  • Rats
  • Receptor, Muscarinic M1
  • Receptors, Muscarinic / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Tyrosine / metabolism*
  • Xenopus laevis


  • Actins
  • CTTN protein, human
  • Cortactin
  • Cttn protein, rat
  • KCNA2 protein, human
  • Kcna2 protein, rat
  • Kv1.2 Potassium Channel
  • Microfilament Proteins
  • Potassium Channels
  • Potassium Channels, Voltage-Gated
  • Receptor, Muscarinic M1
  • Receptors, Muscarinic
  • Recombinant Fusion Proteins
  • Tyrosine