2-aminoethoxydiphenyl borate (2-APB) is a reliable blocker of store-operated Ca2+ entry but an inconsistent inhibitor of InsP3-induced Ca2+ release

FASEB J. 2002 Aug;16(10):1145-50. doi: 10.1096/fj.02-0037rev.


Since its introduction to Ca2+ signaling in 1997, 2-aminoethoxydiphenyl borate (2-APB) has been used in many studies to probe for the involvement of inositol 1,4,5-trisphosphate receptors in the generation of Ca2+ signals. Due to reports of some nonspecific actions of 2-APB, and the fact that its principal antagonistic effect is on Ca2+ entry rather than Ca2+ release, this compound may not have the utility first suggested. However, 2-APB has thrown up some interesting results, particularly with respect to store-operated Ca2+ entry in nonexcitable cells. These data indicate that although it must be used with caution, 2-APB can be useful in probing certain aspects of Ca2+ signaling.

Publication types

  • Review

MeSH terms

  • Animals
  • Boron Compounds / pharmacology*
  • Calcium / metabolism*
  • Calcium Channel Blockers / pharmacology*
  • Calcium Channels / metabolism
  • Calcium Signaling / drug effects
  • Inositol 1,4,5-Trisphosphate / antagonists & inhibitors*
  • Inositol 1,4,5-Trisphosphate Receptors
  • Ion Transport / drug effects
  • Macrocyclic Compounds
  • Mice
  • Oxazoles / pharmacology
  • Receptors, Cytoplasmic and Nuclear / metabolism


  • Boron Compounds
  • Calcium Channel Blockers
  • Calcium Channels
  • Inositol 1,4,5-Trisphosphate Receptors
  • Macrocyclic Compounds
  • Oxazoles
  • Receptors, Cytoplasmic and Nuclear
  • xestospongin A
  • Inositol 1,4,5-Trisphosphate
  • 2-aminoethoxydiphenyl borate
  • Calcium