Three-dimensional reconstructions of extracellular matrix polymers using automated electron tomography

J Struct Biol. 2002 Apr-May;138(1-2):130-6. doi: 10.1016/s1047-8477(02)00028-x.

Abstract

The extracellular matrix is an intricate network of macromolecules which provides support for cells and a framework for tissues. The detailed structure and organisation of most matrix polymers is poorly understood. These polymers have a complex ultrastructure, and it has proved a major challenge both to define their structural organisation and to relate this to their biological function. However, new approaches using automated electron tomography are beginning to reveal important insights into the molecular assembly and structural organisation of two of the most abundant polymer systems in the extracellular matrix. We have generated three-dimensional reconstructions of collagen fibrils from bovine cornea and fibrillin microfibrils from ciliary zonules. Analysis of these data has provided new insights into the organisation and function of these large macromolecular assemblies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cornea / ultrastructure
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / ultrastructure*
  • Fibrillar Collagens / chemistry
  • Fibrillar Collagens / ultrastructure
  • Fibrillins
  • Imaging, Three-Dimensional / methods*
  • Microfibrils / chemistry
  • Microfibrils / ultrastructure
  • Microfilament Proteins / chemistry
  • Microfilament Proteins / ultrastructure
  • Microscopy, Electron / methods
  • Tomography, X-Ray Computed / methods*

Substances

  • Fibrillar Collagens
  • Fibrillins
  • Microfilament Proteins