Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling

Neuron. 2002 Jul 18;35(2):255-65. doi: 10.1016/s0896-6273(02)00751-1.

Abstract

Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tlc, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tlc. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Patterning / genetics*
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins / genetics
  • Bone Morphogenetic Proteins / metabolism
  • Cell Differentiation / genetics
  • Cell Lineage / genetics
  • Cytoskeletal Proteins
  • Denervation
  • Gastrula / cytology
  • Gastrula / metabolism*
  • Gene Expression Regulation / physiology*
  • Immunohistochemistry
  • Mesencephalon / cytology
  • Mesencephalon / embryology
  • Mesencephalon / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Neurons / cytology
  • Neurons / metabolism
  • Phylogeny
  • Prosencephalon / cytology
  • Prosencephalon / embryology
  • Prosencephalon / metabolism
  • Proteins / genetics
  • Proteins / isolation & purification*
  • Proteins / metabolism
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Signal Transduction / genetics
  • Telencephalon / cytology
  • Telencephalon / embryology*
  • Telencephalon / metabolism
  • Transforming Growth Factor beta*
  • Wnt Proteins
  • Zebrafish / embryology*
  • Zebrafish / genetics
  • Zebrafish / metabolism
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / isolation & purification*

Substances

  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins
  • Cytoskeletal Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • Transforming Growth Factor beta
  • Wnt Proteins
  • Zebrafish Proteins
  • bmp7a protein, zebrafish
  • frizzled related protein-1
  • wnt8a protein, zebrafish
  • wnt8b protein, zebrafish

Associated data

  • GENBANK/AF520426