Combinatorial expression of TRPV channel proteins defines their sensory functions and subcellular localization in C. elegans neurons

Neuron. 2002 Jul 18;35(2):307-18. doi: 10.1016/s0896-6273(02)00757-2.

Abstract

C. elegans OSM-9 is a TRPV channel protein involved in sensory transduction and adaptation. Here, we show that distinct sensory functions arise from different combinations of OSM-9 and related OCR TRPV proteins. Both OSM-9 and OCR-2 are essential for several forms of sensory transduction, including olfaction, osmosensation, mechanosensation, and chemosensation. In neurons that express both OSM-9 and OCR-2, tagged OCR-2 and OSM-9 proteins reside in sensory cilia and promote each other's localization to cilia. In neurons that express only OSM-9, tagged OSM-9 protein resides in the cell body and acts in sensory adaptation rather than sensory transduction. Thus, alternative combinations of TRPV proteins may direct different functions in distinct subcellular locations. Animals expressing the mammalian TRPV1 (VR1) channel in ASH nociceptor neurons avoid the TRPV1 ligand capsaicin, allowing selective, drug-inducible activation of a specific behavior.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Base Sequence / genetics
  • Behavior, Animal / drug effects
  • Behavior, Animal / physiology
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / isolation & purification*
  • Caenorhabditis elegans Proteins / metabolism
  • Capsaicin / pharmacology
  • Cell Compartmentation / genetics
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Gene Expression Regulation / physiology
  • Ion Channels / genetics
  • Ion Channels / isolation & purification*
  • Ion Channels / metabolism*
  • Ion Channels / ultrastructure
  • Molecular Sequence Data
  • Mutation / genetics
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification*
  • Nerve Tissue Proteins / metabolism*
  • Nerve Tissue Proteins / ultrastructure
  • Nervous System / cytology
  • Nervous System / drug effects
  • Nervous System / metabolism*
  • Neurons, Afferent / cytology
  • Neurons, Afferent / drug effects
  • Neurons, Afferent / metabolism*
  • Pain / genetics
  • Pain / metabolism
  • Pain / physiopathology
  • Phylogeny
  • Receptors, Drug / drug effects
  • Receptors, Drug / metabolism
  • Receptors, Drug / ultrastructure
  • Sensation / drug effects
  • Sensation / genetics*
  • Signal Transduction / genetics
  • TRPV Cation Channels
  • Transient Receptor Potential Channels

Substances

  • Caenorhabditis elegans Proteins
  • Ion Channels
  • Nerve Tissue Proteins
  • OCR-1 protein, C elegans
  • OCR-2 protein, C elegans
  • OSM-9 protein, C elegans
  • Receptors, Drug
  • TRPV Cation Channels
  • Transient Receptor Potential Channels
  • Capsaicin