Differential trafficking of the vesicular monoamine transporter-2 by methamphetamine and cocaine

Eur J Pharmacol. 2002 Aug 2;449(1-2):71-4. doi: 10.1016/s0014-2999(02)01985-4.


High-dose administration of cocaine or methamphetamine to rats acutely (< or = 24 h) alters vesicular dopamine transport. This study elucidates the nature of these changes. Results reveal a differential redistribution of the vesicular monoamine transporter-2 (VMAT-2) within striatal synaptic terminals after drug treatment. In particular, cocaine shifts VMAT-2 protein from a synaptosomal membrane fraction to a vesicle-enriched fraction, as assessed ex vivo in fractions prepared from treated rats. In contrast, methamphetamine treatment redistributes VMAT-2 from a vesicle-enriched fraction to a location that is not retained in a synaptosomal preparation. These data suggest that psychostimulants acutely and differentially affect VMAT-2 subcellular localization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Central Nervous System Stimulants / pharmacology*
  • Cocaine / pharmacology*
  • Male
  • Membrane Glycoproteins / metabolism*
  • Membrane Transport Proteins*
  • Methamphetamine / pharmacology*
  • Neostriatum / drug effects
  • Neostriatum / metabolism
  • Neuropeptides*
  • Presynaptic Terminals / drug effects
  • Presynaptic Terminals / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Synaptic Vesicles / drug effects
  • Synaptic Vesicles / metabolism
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins


  • Central Nervous System Stimulants
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Neuropeptides
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins
  • Methamphetamine
  • Cocaine