The effects of vitamins E and C on fracture healing in rats

J Int Med Res. May-Jun 2002;30(3):309-13. doi: 10.1177/147323000203000312.


Forty-eight rats were divided into four groups of 12. For 3 days, each group received the following vitamins in once-daily doses: group 1, vitamin E only; group 2, vitamin C only; group 3, vitamins E and C; and group 4, no treatment (control). The right tibia of each rat was fractured on day 4; the rats then received the same regimen three times a week (once-daily doses) until day 21. Fracture healing was evaluated radiologically by measuring the callus indices, and histologically by a 5-point grading scale. On days 14 and 21, the callus index values in group 2 were statistically higher than those in the other groups. Histological evaluation scores in group 2 were the highest overall, and group 3 scores were higher than those in groups 1 and 4. These findings indicate that vitamin C accelerates fracture healing, vitamin E does not exert a marked effect on this process, and vitamins E and C in combination do not have a synergistic impact on fracture healing.

MeSH terms

  • Animals
  • Ascorbic Acid / pharmacology*
  • Fracture Healing / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Vitamin E / pharmacology*


  • Vitamin E
  • Ascorbic Acid