Effects of dexamethasone on mucin gene expression in cultured human nasal epithelial cells

Laryngoscope. 2002 Aug;112(8 Pt 1):1436-40. doi: 10.1097/00005537-200208000-00020.


Objective: To clarify 1) which mucin gene expression is influenced by glucocorticoid and 2) whether glucocorticoid influences steady-state mucin expression or mucin gene expression induced by lipopolysaccharide.

Methods: Dissociated cells obtained from nasal polyps were cultured on a collagen gel substrate in an air-liquid interface. Dexamethasone was added to the culture medium in the steady-state and prior to the stimulation by LPS. RNAs were extracted from culture cells, and semiquantitative reverse transcriptase-polymerase chain reaction was performed for MUC2, MUC5AC, MUC5B, MUC8, and beta-actin.

Results: Dexamethasone did not influence steady-state messenger RNA levels of either mucin gene. Dexamethasone suppressed lipopolysaccharide-induced messenger RNA levels of MUC8.

Conclusion: Glucocorticoid does not influence steady-state mucin gene expression but suppresses mucin gene expression induced by secretagogues such as lipopolysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Dexamethasone / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Glucocorticoids / pharmacology*
  • Humans
  • Lipopolysaccharides / pharmacology
  • Mucins / drug effects*
  • Mucins / genetics*
  • Nasal Mucosa / cytology*
  • Nasal Mucosa / drug effects*


  • Glucocorticoids
  • Lipopolysaccharides
  • Mucins
  • Dexamethasone