A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings

Planta. 2002 Aug;215(4):549-56. doi: 10.1007/s00425-002-0787-4. Epub 2002 Jul 4.

Abstract

Jasmonates (JAs) regulate Arabidopsis thaliana (L.) Heynh. wound and defense responses, pollen development, and stress-related growth inhibition. Significantly, each of these responses requires COI1, an F-box protein. We fused firefly luciferase as a reporter to the JA-responsive promoter for the vegetative storage protein gene (VSP) and used this to screen for mutants that failed to express luciferase in the presence of JA, isolating a mutant designated coi1-16. Comparisons with coi1-1 and jar1-1 plants indicated that coi1-16 was only slightly more sensitive to JA than coi1-1 plants. However, whilst coi1-16 plants failed to produce viable pollen at 22 degrees C, they were fertile at 16 degrees C. Therefore, unlike the other coi1 mutants, coi1-16 could be maintained as a pure line and did not require selection. We have used coi1-16 seeds to define novel interactions between JA and other hormone signalling pathways in seed germination and in the development of young seedlings.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Acetates / pharmacology
  • Alleles
  • Amino Acid Substitution
  • Animals
  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Cyclopentanes / pharmacology
  • Darkness
  • Endopeptidases / genetics
  • Endopeptidases / metabolism
  • Ethylenes / pharmacology
  • Fertility / drug effects
  • Fertility / genetics
  • Hypocotyl / drug effects
  • Hypocotyl / genetics
  • Hypocotyl / growth & development
  • Leucine / genetics
  • Mutation
  • Mutation, Missense
  • Nucleotidyltransferases / genetics
  • Nucleotidyltransferases / metabolism
  • Oxylipins
  • Phenylalanine / genetics
  • Plant Growth Regulators / pharmacology*
  • Plant Roots / drug effects
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Structures / drug effects
  • Plant Structures / genetics
  • Plant Structures / growth & development*
  • Plants, Genetically Modified
  • Pollen / drug effects
  • Pollen / genetics
  • Pollen / growth & development
  • Promoter Regions, Genetic / genetics
  • Repetitive Sequences, Amino Acid / genetics
  • Seeds / drug effects
  • Seeds / genetics
  • Seeds / growth & development
  • Signal Transduction / drug effects
  • Signal Transduction / genetics
  • Signal Transduction / physiology*
  • Sodium Chloride / pharmacology
  • Stress, Mechanical
  • Temperature
  • Time Factors

Substances

  • Acetates
  • Arabidopsis Proteins
  • COI1 protein, Arabidopsis
  • Cyclopentanes
  • Ethylenes
  • Oxylipins
  • Plant Growth Regulators
  • THI1 protein, Arabidopsis
  • VSP2 protein, Arabidopsis
  • Sodium Chloride
  • Phenylalanine
  • Abscisic Acid
  • methyl jasmonate
  • ethylene
  • JAR1 protein, Arabidopsis
  • Nucleotidyltransferases
  • VSP1 protein, Arabidopsis
  • Endopeptidases
  • Leucine