Abstract
Jasmonates (JAs) regulate Arabidopsis thaliana (L.) Heynh. wound and defense responses, pollen development, and stress-related growth inhibition. Significantly, each of these responses requires COI1, an F-box protein. We fused firefly luciferase as a reporter to the JA-responsive promoter for the vegetative storage protein gene (VSP) and used this to screen for mutants that failed to express luciferase in the presence of JA, isolating a mutant designated coi1-16. Comparisons with coi1-1 and jar1-1 plants indicated that coi1-16 was only slightly more sensitive to JA than coi1-1 plants. However, whilst coi1-16 plants failed to produce viable pollen at 22 degrees C, they were fertile at 16 degrees C. Therefore, unlike the other coi1 mutants, coi1-16 could be maintained as a pure line and did not require selection. We have used coi1-16 seeds to define novel interactions between JA and other hormone signalling pathways in seed germination and in the development of young seedlings.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Acetates / pharmacology
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Alleles
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Amino Acid Substitution
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Animals
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Arabidopsis / drug effects
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Cyclopentanes / pharmacology
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Darkness
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Endopeptidases / genetics
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Endopeptidases / metabolism
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Ethylenes / pharmacology
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Fertility / drug effects
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Fertility / genetics
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Hypocotyl / drug effects
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Hypocotyl / genetics
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Hypocotyl / growth & development
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Leucine / genetics
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Mutation
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Mutation, Missense
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Nucleotidyltransferases / genetics
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Nucleotidyltransferases / metabolism
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Oxylipins
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Phenylalanine / genetics
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Plant Growth Regulators / pharmacology*
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Plant Roots / drug effects
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Plant Roots / genetics
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Plant Roots / growth & development
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Plant Structures / drug effects
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Plant Structures / genetics
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Plant Structures / growth & development*
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Plants, Genetically Modified
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Pollen / drug effects
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Pollen / genetics
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Pollen / growth & development
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Promoter Regions, Genetic / genetics
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Repetitive Sequences, Amino Acid / genetics
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Seeds / drug effects
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Seeds / genetics
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Seeds / growth & development
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Signal Transduction / physiology*
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Sodium Chloride / pharmacology
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Stress, Mechanical
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Temperature
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Time Factors
Substances
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Acetates
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Arabidopsis Proteins
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COI1 protein, Arabidopsis
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Cyclopentanes
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Ethylenes
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Oxylipins
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Plant Growth Regulators
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THI1 protein, Arabidopsis
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VSP2 protein, Arabidopsis
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Sodium Chloride
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Phenylalanine
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Abscisic Acid
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methyl jasmonate
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ethylene
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JAR1 protein, Arabidopsis
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Nucleotidyltransferases
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VSP1 protein, Arabidopsis
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Endopeptidases
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Leucine