Differential Pax6 promoter activity and transcript expression during forebrain development

Mech Dev. 2002 Jun;114(1-2):171-5. doi: 10.1016/s0925-4773(02)00051-5.

Abstract

Three different Pax6 promoters -- P0, P1, and P alpha -- show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse transcription-polymerase chain reaction to detect transcripts initiated from each promoter. Promoter-specific transcripts are expressed within subdomains of total Pax6 expression, but differ from one another in their spatial localization and expression over time. Additionally, we identified a novel P0-initiated transcript and detected a developmentally regulated antisense transcript.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Animals
  • Base Sequence
  • Eye Proteins
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins / genetics*
  • In Situ Hybridization
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Promoter Regions, Genetic*
  • Prosencephalon / embryology*
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors

Substances

  • Eye Proteins
  • Homeodomain Proteins
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Pax6 protein, mouse
  • Repressor Proteins