Platelet-derived growth factor-B and -C and active alpha-receptors in medulloblastoma cells

Biochem Biophys Res Commun. 2002 Aug 23;296(3):604-11. doi: 10.1016/s0006-291x(02)00917-8.


The malignant childhood brain tumor medulloblastoma belongs to the group of primitive neuroectodermal tumours (PNETs). Medulloblastomas are thought to arise from remnants of the transient external germinal layer in the cerebellum. Proliferation, differentiation, and motility of cells in the central nervous system are regulated by growth factors, e.g., platelet-derived growth factor (PDGF). Recently, it was shown that higher level of PDGF alpha-receptor expression is characteristic of metastatic medulloblastomas. We have investigated five medulloblastoma/PNET cell lines and found that the PDGF alpha-receptor is actively signalling in most of them, an activity most likely driven by endogenously produced PDGF-C. PDGF-C is normally present in cells of the developing external germinal layer and our results are consistent with the idea that medulloblastomas are derived from such cells undergoing early neuronal differentiation. Moreover, the expression of PDGF and its receptors was associated with neuronal characteristics, but not with high levels of c-myc expression in the medullablastoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cells, Cultured
  • Cerebellar Neoplasms / genetics
  • Cerebellar Neoplasms / metabolism*
  • DNA, Neoplasm / biosynthesis
  • Dose-Response Relationship, Drug
  • Humans
  • Lymphokines
  • Medulloblastoma / genetics
  • Medulloblastoma / metabolism*
  • Neurons / cytology
  • Neurons / metabolism
  • Platelet-Derived Growth Factor / biosynthesis*
  • Platelet-Derived Growth Factor / genetics
  • Platelet-Derived Growth Factor / pharmacology
  • Proto-Oncogene Proteins c-myc / biosynthesis
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-sis / biosynthesis*
  • Proto-Oncogene Proteins c-sis / genetics
  • RNA, Neoplasm / biosynthesis
  • Receptor, Platelet-Derived Growth Factor alpha / genetics
  • Receptor, Platelet-Derived Growth Factor alpha / metabolism*
  • Tumor Cells, Cultured


  • DNA, Neoplasm
  • Lymphokines
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins c-sis
  • RNA, Neoplasm
  • platelet-derived growth factor C
  • Receptor, Platelet-Derived Growth Factor alpha