Parallel Quorum Sensing Systems Converge to Regulate Virulence in Vibrio Cholerae

Cell. 2002 Aug 9;110(3):303-14. doi: 10.1016/s0092-8674(02)00829-2.

Abstract

The marine bacterium Vibrio harveyi possesses two quorum sensing systems (System 1 and System 2) that regulate bioluminescence. Although the Vibrio cholerae genome sequence reveals that a V. harveyi-like System 2 exists, it does not predict the existence of a V. harveyi-like System 1 or any obvious quorum sensing-controlled target genes. In this report we identify and characterize the genes encoding an additional V. cholerae autoinducer synthase and its cognate sensor. Analysis of double mutants indicates that a third as yet unidentified sensory circuit exists in V. cholerae. This quorum sensing apparatus is unusually complex, as it is composed of at least three parallel signaling channels. We show that in V. cholerae these communication systems converge to control virulence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / genetics
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification*
  • Cell Communication / genetics*
  • DNA, Bacterial / genetics
  • Gene Expression Regulation, Bacterial / genetics*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Signal Transduction / genetics*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Vibrio cholerae / genetics*
  • Vibrio cholerae / pathogenicity*
  • Virulence / genetics

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Luminescent Proteins
  • LuxU protein, Vibrio
  • Phosphoproteins
  • Repressor Proteins
  • Trans-Activators
  • LuxR autoinducer binding proteins
  • N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
  • 4-Butyrolactone