Nucleotide sequences of the internal transcribed spacers and 5.8S region of nuclear ribosomal DNA in Pinus taeda L. and Pinus echinata Mill

Jiwang Chen; Charles G Tauer; Yinghua Huang

doi:10.1080/10425170290030060

Nucleotide sequences of the internal transcribed spacers and 5.8S region of nuclear ribosomal DNA in Pinus taeda L. and Pinus echinata Mill

DNA Seq. 2002 Apr;13(2):129-31. doi: 10.1080/10425170290030060.

Authors

Jiwang Chen¹, Charles G Tauer, Yinghua Huang

Affiliation

¹ 008 Room C, Agricultural Hall, Forestry Genetics Laboratory, Oklahoma State University, Stillwater, OK 74078, USA. chenjiw@hotmail.com

PMID: 12180347
DOI: 10.1080/10425170290030060

Abstract

The 615-bp nucleotide sequences of the first partial internal transcribed spacer (ITS-1), 5.8S rDNA and ITS-2 region from Pinus taeda L. (loblolly pine) and Pinus echinata Mill. (shortleaf pine) are reported. The two pine species show the same nucleotide sequences in this region, which indicates their close phylogenetic relationship. However, our reported nucleotide sequence in this region from shortleaf pine is different from the previous report for shortleaf pine in GenBank (accession number: AF037016). Our PCR-RFLP analysis of this region confirms our sequencing data. This correction is important for pine phylogenetic studies because it is located in the conserved 5.8S rDNA region.

MeSH terms

Amino Acid Sequence
DNA, Ribosomal*
Molecular Sequence Data
Pinus / genetics*
Pinus taeda
RNA, Ribosomal, 5.8S / genetics*

Substances

DNA, Ribosomal
RNA, Ribosomal, 5.8S

Associated data

GENBANK/AF367378
GENBANK/AF367379