Echocardiographic assessment of cardiac function in diabetic db/db and transgenic db/db-hGLUT4 mice

Am J Physiol Heart Circ Physiol. 2002 Sep;283(3):H976-82. doi: 10.1152/ajpheart.00088.2002.

Abstract

Control db/+ and diabetic db/db mice at 6 and 12 wk of age were subjected to echocardiography to determine whether contractile function was reduced in vivo and restored in transgenic db/db-human glucose transporter 4 (hGLUT4) mice (12 wk old) in which cardiac metabolism has been normalized. Systolic function was unchanged in 6-wk-old db/db mice, but fractional shortening and velocity of circumferential fiber shortening were reduced in 12-wk-old db/db mice (43.8 +/- 2.1% and 8.3 +/- 0.5 circs/s, respectively) relative to db/+ control mice (59.5 +/- 2.3% and 11.8 +/- 0.4 circs/s, respectively). Doppler flow measurements were unchanged in 6-wk-old db/db mice. The ratio of E and A transmitral flows was reduced from 3.56 +/- 0.29 in db/+ mice to 2.40 +/- 0.20 in 12-wk-old db/db mice, indicating diastolic dysfunction. Thus a diabetic cardiomyopathy with systolic and diastolic dysfunction was evident in 12-wk-old diabetic db/db mice. Cardiac function was normalized in transgenic db/db-hGLUT4 mice, indicating that altered cardiac metabolism can produce contractile dysfunction in diabetic db/db hearts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiomyopathies / diagnostic imaging*
  • Cardiomyopathies / etiology
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / diagnostic imaging*
  • Diastole / physiology*
  • Echocardiography / standards
  • Echocardiography / statistics & numerical data
  • Glucose Transporter Type 4
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Monosaccharide Transport Proteins / genetics*
  • Muscle Proteins*
  • Observer Variation
  • Reproducibility of Results
  • Systole / physiology*

Substances

  • Glucose Transporter Type 4
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Slc2a4 protein, mouse