The calicheamicin gene cluster and its iterative type I enediyne PKS

Science. 2002 Aug 16;297(5584):1173-6. doi: 10.1126/science.1072105.


The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents / biosynthesis*
  • Antibiotics, Antineoplastic / biosynthesis*
  • Blotting, Southern
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • Conserved Sequence
  • Enediynes
  • Genes, Bacterial*
  • Micromonospora / enzymology
  • Micromonospora / genetics*
  • Micromonospora / metabolism
  • Multienzyme Complexes / chemistry*
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism
  • Multigene Family
  • Mutation
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA


  • Aminoglycosides
  • Anti-Bacterial Agents
  • Antibiotics, Antineoplastic
  • Enediynes
  • Multienzyme Complexes
  • calicheamicin gamma(1)I
  • C 1027