TIP49, but not TRRAP, modulates c-Myc and E2F1 dependent apoptosis

Oncogene. 2002 Aug 29;21(38):5835-43. doi: 10.1038/sj.onc.1205763.

Abstract

We previously described two nuclear cofactors, TRRAP and TIP49, that have functional roles in Myc-mediated oncogenesis. We have now expanded the analysis of these Myc-associated cofactors to investigate their roles in apoptosis and cell proliferation. Although TRRAP and TIP49 are both essential for transformation, TIP49 modulates c-Myc-mediated apoptosis whereas disruption of TRRAP activity has no apparent effect on apoptosis. We extended our analysis of TIP49 to show that it also binds to the E2F1 transactivation domain and modulates both transforming and apoptotic activities. These results indicate that individual cofactors differentially potentiate c-Myc and E2F1 functions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Adaptor Proteins, Signal Transducing
  • Adenovirus E1A Proteins / genetics
  • Adenovirus E1A Proteins / metabolism
  • Animals
  • Apoptosis / physiology*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Cycle / genetics
  • Cell Cycle Proteins*
  • Cell Division / genetics
  • Cell Transformation, Neoplastic / genetics
  • Cells, Cultured
  • DNA Helicases*
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Fibroblasts / pathology
  • Genes, Dominant
  • Humans
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Proto-Oncogene Proteins c-myc / genetics*
  • Proto-Oncogene Proteins c-myc / metabolism
  • Rats
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Adenovirus E1A Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • E2f1 protein, rat
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-myc
  • Transcription Factors
  • transformation-transcription domain-associated protein
  • ATPases Associated with Diverse Cellular Activities
  • DNA Helicases
  • RUVBL1 protein, human
  • RUVBL2 protein, human
  • Ruvbl1 protein, rat