Identification of dehydroepiandrosterone metabolites formed from human prostate homogenate using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry

J Chromatogr A. 2002 Jun 28;961(1):97-105. doi: 10.1016/s0021-9673(02)00134-6.


The identification of the in vitro metabolites of dehydroepiandrosterone formed from human prostate homogenate was investigated by hyphenated techniques using the stable-isotope dilution method. A mixture of dehydroepiandrosterone and [2H4]dehydroepiandrosterone was incubated with hypertrophied human prostate tissue homogenate in the presence of NAD, NADH and NADPH. The metabolites were extracted with AcOEt-hexane, purified by solid-phase extraction, and then analyzed by LC-atmospheric pressure chemical ionization MS and/or GC-MS. Androst-5-ene-3beta,17beta-diol (major product), androst-4-ene-3,17-dione, testosterone, 5alpha-dihydrotestosterone, androsterone, and 7alpha-hydroxydehydroepiandrosterone were identified in comparison with authentic samples based on their chromatographic behavior and mass spectra.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Gas / methods*
  • Chromatography, Liquid / methods*
  • Dehydroepiandrosterone / metabolism*
  • Humans
  • Male
  • Mass Spectrometry / methods*
  • Prostate / metabolism*


  • Dehydroepiandrosterone