D-RNAi (messenger RNA-antisense DNA interference) as a novel defense system against cancer and viral infections

Curr Cancer Drug Targets. 2001 Nov;1(3):241-7. doi: 10.2174/1568009013334151.


D-RNAi (Messenger RNA-antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4(+) T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99% endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.

Publication types

  • Review

MeSH terms

  • DNA, Antisense / pharmacology
  • DNA, Complementary / genetics
  • DNA, Complementary / metabolism*
  • Gene Silencing*
  • Humans
  • Neoplasms / genetics*
  • Neoplasms / therapy
  • RNA Processing, Post-Transcriptional / genetics
  • RNA Processing, Post-Transcriptional / physiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • RNA, Small Interfering
  • RNA, Untranslated / genetics*
  • RNA, Untranslated / metabolism*
  • Transcription, Genetic


  • DNA, Antisense
  • DNA, Complementary
  • RNA, Messenger
  • RNA, Small Interfering
  • RNA, Untranslated