Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissues: effect of vanadate and fenugreek (Trigonellafoenum graecum)

Mol Cell Biochem. 2002 Jul;236(1-2):7-12. doi: 10.1023/a:1016103131408.


With the premise that oxygen free radicals may be responsible for the severity and complications of diabetes, the level of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as the oxidative damage were examined in the tissues of control, diabetic and treated rats. After 3 weeks of diabetes, the activity of CAT was significantly increased in heart in diabetes (about 6-fold) but decreased in liver. The SOD activity decreased significantly in liver but increased in brain. The activity of GPx decreased significantly in liver and increased in kidney. A significant increase was observed in oxidative damage in heart and kidney and a small increase in brain with decrease in liver and muscle. Vanadate and fenugreek (Trigonella foenum graecum) administration to diabetic animals showed a reversal of the disturbed antioxidant levels and peroxidative damage. Results suggest that oxidative stress play a key role in the complications of diabetes. Vanadate and fenugreek seeds showed an encouraging antioxidant property and can be valuable candidates in the treatment of the reversal of the complications of diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloxan
  • Animals
  • Antioxidants / metabolism*
  • Body Weight / drug effects
  • Brain / enzymology
  • Catalase / biosynthesis
  • Diabetes Mellitus, Experimental / metabolism*
  • Female
  • Glutathione Peroxidase / biosynthesis
  • Insulin / pharmacology
  • Kidney / enzymology
  • Lipid Peroxidation
  • Liver / enzymology
  • Organ Size / drug effects
  • Oxidative Stress*
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / biosynthesis
  • Time Factors
  • Trigonella / metabolism*
  • Vanadates / pharmacology*


  • Antioxidants
  • Insulin
  • Plant Extracts
  • Vanadates
  • Alloxan
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase