The skin fungus-induced Th1- and Th2-related cytokine, chemokine and prostaglandin E2 production in peripheral blood mononuclear cells from patients with atopic dermatitis and psoriasis vulgaris

Clin Exp Allergy. 2002 Aug;32(8):1243-50. doi: 10.1046/j.1365-2745.2002.01459.x.

Abstract

Background: It is suggested that skin fungi may be involved in the development of atopic dermatitis (AD) and psoriasis vulgaris (PV).

Objective: We studied skin fungus-induced Th1- or Th2-related cytokine, chemokine and prostaglandin E2 (PGE2) secretion in peripheral blood mononuclear cells (PBMC) from patients with AD and PV and normal subjects.

Methods: PBMC were cultured with the extracts of Malassezia furfur (MF), Candida albicans (CA) and Trichophyton rubrum (TR). The cytokine, chemokine and PGE2 amounts in the supernatants were measured by enzyme-linked immunosorbent assays.

Results: MF induced IL-4 and macrophage-derived chemokine (MDC) secretion in AD patients, while induced IFN-gamma and interferon-inducible protein of 10 kDa (IP-10) secretion in PV patients, however, did not induce either secretion in normal subjects. CA induced IL-4, MDC, IFN-gamma and IP-10 secretion in AD and PV patients and normal subjects. In AD patients, the magnitude of IL-4 and MDC responses to CA was higher than that to MF. Compared with PV patients and normal subjects, the magnitude of IL-4 and MDC responses to CA was higher while that of IFN-gamma and IP-10 responses to CA was lower in AD patients. TR induced moderate IL-4 and MDC secretion only in AD patients. The three fungi induced higher levels of PGE2 secretion in AD patients than in PV patients and normal subjects. Cyclooxygenase-2 inhibitor NS-398 suppressed PGE2 responses to MF, CA and TR, and partially suppressed IL-4 and MDC responses to MF, CA and TR, while enhanced IFN-gamma and IP-10 responses to CA in AD patients, and these effects of NS-398 were reversed by cyclic AMP analogue.

Conclusion: AD patients manifest Th2-skewed responses to MF, CA and TR, which may be partially attributable to the enhanced PGE2 responses to these fungi. PV patients manifest Th1-skewed responses to MF.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antigens, Fungal / adverse effects*
  • Candida albicans / immunology
  • Case-Control Studies
  • Chemokine CCL22
  • Chemokine CXCL10
  • Chemokines, CC / immunology
  • Chemokines, CXC / immunology
  • Cyclooxygenase Inhibitors / pharmacology
  • Dermatitis, Atopic / immunology
  • Dermatitis, Atopic / microbiology*
  • Dermatomycoses / immunology*
  • Dinoprostone / metabolism
  • Female
  • Humans
  • Indomethacin / pharmacology
  • Interferon-gamma / immunology
  • Interleukin-4 / immunology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Malassezia / immunology*
  • Male
  • Nitrobenzenes / pharmacology
  • Psoriasis / immunology
  • Psoriasis / microbiology*
  • Pyrazoles / pharmacology
  • Sulfonamides / pharmacology
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Trichophyton / immunology

Substances

  • Antigens, Fungal
  • CCL22 protein, human
  • Chemokine CCL22
  • Chemokine CXCL10
  • Chemokines, CC
  • Chemokines, CXC
  • Cyclooxygenase Inhibitors
  • Nitrobenzenes
  • Pyrazoles
  • SC 560
  • Sulfonamides
  • N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
  • Interleukin-4
  • Interferon-gamma
  • Dinoprostone
  • Indomethacin