Memory for context is impaired by injecting anisomycin into dorsal hippocampus following context exploration

Behav Brain Res. 2002 Aug 21;134(1-2):299-306. doi: 10.1016/s0166-4328(02)00045-1.


Pre-exposure to the context facilitates the small amount of contextual fear conditioning that is normally produced by immediate shock. This context pre-exposure facilitation effect provides a convenient way to study the rat's learning about context. We recently reported that anterograde damage to dorsal hippocampus prevents this facilitation. The present experiments strengthen this conclusion by showing that the protein synthesis inhibitor, anisomycin, injected bilaterally into the dorsal hippocampus following context pre-exposure also significantly reduces the facilitation effect. The same treatment given immediately after immediate shock, however, had no effect on facilitation. These results support theories that assume that, (a) contextual fear involves two processes, acquiring and storing a conjunctive representation of a context and associating that representation with fear; and (b) the hippocampus contributes to contextual fear by participating in the storage of the memory representation of the context.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anisomycin / administration & dosage
  • Anisomycin / pharmacology*
  • Exploratory Behavior / drug effects
  • Exploratory Behavior / physiology*
  • Hippocampus / physiology*
  • Injections, Intraventricular
  • Male
  • Memory / drug effects*
  • Microinjections
  • Protein Synthesis Inhibitors / administration & dosage
  • Protein Synthesis Inhibitors / pharmacology*
  • Rats
  • Rats, Long-Evans


  • Protein Synthesis Inhibitors
  • Anisomycin