Redundant role of tissue-selective TAF(II)105 in B lymphocytes

Mol Cell Biol. 2002 Sep;22(18):6564-72. doi: 10.1128/MCB.22.18.6564-6572.2002.

Abstract

Regulated gene expression is a complex process achieved through the function of multiple protein factors acting in concert at a given promoter. The transcription factor TFIID is a central component of the machinery regulating mRNA synthesis by RNA polymerase II. This large multiprotein complex is composed of the TATA box binding protein (TBP) and several TBP-associated factors (TAF(II)s). The recent discovery of multiple TBP-related factors and tissue-specific TAF(II)s suggests the existence of specialized TFIID complexes that likely play a critical role in regulating transcription in a gene- and tissue-specific manner. The tissue-selective factor TAF(II)105 was originally identified as a component of TFIID derived from a human B-cell line. In this report we demonstrate the specific induction of TAF(II)105 in cultured B cells in response to bacterial lipopolysaccharide (LPS). To examine the in vivo role of TAF(II)105, we have generated TAF(II)105-null mice by homologous recombination. Here we show that B-lymphocyte development is largely unaffected by the absence of TAF(II)105. TAF(II)105-null B cells can proliferate in response to LPS, produce relatively normal levels of resting antibodies, and can mount an immune response by producing antigen-specific antibodies in response to immunization. Taken together, we conclude that the function of TAF(II)105 in B cells is likely redundant with the function of other TAF(II)105-related cellular proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism*
  • Blotting, Western
  • Cell Division
  • Cell Separation
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Dose-Response Relationship, Drug
  • Erythrocytes / metabolism
  • Flow Cytometry
  • Immunoglobulin A / metabolism
  • Immunoglobulin G / metabolism
  • Immunoglobulin M / metabolism
  • Lipopolysaccharides / metabolism
  • Mice
  • Precipitin Tests
  • RNA, Messenger / metabolism
  • Spleen / metabolism
  • Spleen / pathology
  • TATA-Binding Protein Associated Factors*
  • Time Factors
  • Transcription Factor TFIID*
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Transcription, Genetic
  • Transcriptional Activation

Substances

  • DNA-Binding Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Lipopolysaccharides
  • RNA, Messenger
  • TATA-Binding Protein Associated Factors
  • Taf4b protein, mouse
  • Transcription Factor TFIID
  • Transcription Factors