hamlet, a binary genetic switch between single- and multiple- dendrite neuron morphology

Science. 2002 Aug 23;297(5585):1355-8. doi: 10.1126/science.1072387.

Abstract

The dendritic morphology of neurons determines the number and type of inputs they receive. In the Drosophila peripheral nervous system (PNS), the external sensory (ES) neurons have a single nonbranched dendrite, whereas the lineally related multidendritic (MD) neurons have extensively branched dendritic arbors. We report that hamlet is a binary genetic switch between these contrasting morphological types. In hamlet mutants, ES neurons are converted to an MD fate, whereas ectopic hamlet expression in MD precursors results in transformation of MD neurons into ES neurons. Moreover, hamlet expression induced in MD neurons undergoing dendrite outgrowth drastically reduces arbor branching.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Differentiation
  • Cell Lineage
  • Clone Cells
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology*
  • Dendrites / ultrastructure*
  • Drosophila / embryology*
  • Drosophila / genetics
  • Drosophila / physiology
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / physiology*
  • Gene Expression
  • Genetic Complementation Test
  • Mitosis
  • Molecular Sequence Data
  • Morphogenesis
  • Mutation
  • Neurons / physiology
  • Neurons / ultrastructure*
  • Neurons, Afferent / ultrastructure*
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / physiology*
  • Peripheral Nervous System / cytology
  • Peripheral Nervous System / embryology
  • RNA, Double-Stranded / genetics
  • Sense Organs / embryology
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / physiology*

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • HAM protein, Drosophila
  • Nuclear Proteins
  • RNA, Double-Stranded
  • Transcription Factors