Abstract
The mapping of linear epitopes of B virus (Cercopithecine herpesvirus 1 and herpes B virus) glycoprotein D (gD) was accomplished by screening the constructed gD epitope library with serum from B virus-infected macaques. The immunodominant epitope, gD (362-370), was identified within the C-terminal region of B virus gD that was highly conserved among 19 B virus clinical isolates but was not present in either herpes simplex virus (HSV)-1 or HSV-2 gD. A substantial percentage of serum samples from macaques (95%) and humans (80%) infected with B virus contained antibodies to this epitope. Antibodies against HSV types 1 or 2 did not react with this epitope; thus, gD (362-370) has unique potential to detect B virus-specific antibody responses in human serum, even in the presence of antibodies to HSV-1 and HSV-2.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / blood*
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Antibody Specificity
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Antigens, Viral / chemistry
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Antigens, Viral / genetics
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Antigens, Viral / immunology
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Blotting, Western
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Enzyme-Linked Immunosorbent Assay
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Epitope Mapping
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Herpesviridae Infections / diagnosis*
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Herpesviridae Infections / veterinary
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Herpesviridae Infections / virology
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Herpesvirus 1, Cercopithecine / immunology*
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Humans
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Immunodominant Epitopes / chemistry*
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Immunodominant Epitopes / genetics
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Immunodominant Epitopes / immunology*
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Macaca
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Molecular Sequence Data
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Monkey Diseases / diagnosis
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Monkey Diseases / virology
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Neutralization Tests
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Peptides / chemistry
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Peptides / immunology
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Sequence Analysis, DNA
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunodominant Epitopes
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Peptides
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Viral Envelope Proteins
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glycoprotein D, Human herpesvirus 1
Associated data
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GENBANK/AF447074
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GENBANK/AF447075
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GENBANK/AF447076
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GENBANK/AF447077
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GENBANK/AF447078
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GENBANK/AF447079
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GENBANK/AF447080
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GENBANK/AF447081
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GENBANK/AF447082
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GENBANK/AF447083
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GENBANK/AF447084
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GENBANK/AF447085
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GENBANK/AF447086
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GENBANK/AF447087
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GENBANK/AF447088
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GENBANK/AF447089
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GENBANK/AF447090
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GENBANK/AF447091
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GENBANK/AF447092
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GENBANK/AF447093
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GENBANK/AF447094