UCN-01 (7-hydroxystaurosporine) inhibits the growth of human breast cancer xenografts through disruption of signal transduction

Breast Cancer. 2002;9(1):50-4. doi: 10.1007/BF02967547.

Abstract

Background: 7-Hydroxystaurosporine (UCN-01), originally isolated as a phospholipid-dependent protein kinase C inhibitor, has been shown to have antitumor activity against several human cancer cell lines. UCN-01 inhibits cell cycle progression from the G1 to S phase by inhibition of cyclin-dependent kinase (CDK) activity and induction of intrinsic CDK inhibitor protein, leading to dephosphorylation of retinoblastoma (Rb) protein.

Materials and methods: The antitumor activity of UCN-01 has been investigated against three human breast carcinoma strains serially transplanted into nude mice, including estrogen-dependent MCF-7, Br-10, and estrogen-independent MX-1. When the inoculated tumors started growing exponentially, UCN-01 (7.5 mg/kg) was administered intraperitoneally on five consecutive days a week for 2 weeks. The antitumor effect was evaluated as the lowest T/C ratio (%) during the experiments, where T was the relative mean tumor weight of the treated group and C was that of the control group. At the end of UCN-01 administration expression of p21, a protein of the CDK inhibitor family, and phosphorylated and dephosphorylated Rb protein was detected by Western blotting using treated and control tumors.

Results: UCN-01 had activity against MCF-7 and Br-10, with the lowest T/C ratios of 25.0% and 27.0%, respectively, while MX-1 was resistant to UCN-01 with a T/C ratio of 65.9%. The antitumor spectrum of UCN-01 was different from that of other conventional agents such as doxorubicin and cyclophosphamide which were ineffective against Br-10 but were active against MX-1. Although p21 was induced in three tested strains by UCN-01, little dephosphorylated Rb protein was expressed in MX-1 compared with Br-10 and MCF-7 (in vitro).

Conclusion: UCN-01 appeared to be a promising agent for the treatment of breast cancer, with a different mode of action and antitumor spectrum from other currently available antitumor drugs.

MeSH terms

  • Alkaloids / administration & dosage
  • Alkaloids / pharmacology*
  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / pharmacology*
  • Blotting, Western
  • Breast Neoplasms / metabolism*
  • Cyclophosphamide / administration & dosage
  • Cyclophosphamide / pharmacology
  • Dose-Response Relationship, Drug
  • Doxorubicin / administration & dosage
  • Doxorubicin / pharmacology
  • Drug Synergism
  • Estrogens
  • Female
  • Fluorouracil / administration & dosage
  • Fluorouracil / pharmacology
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Neoplasms, Hormone-Dependent / metabolism
  • Protein Kinase C / antagonists & inhibitors*
  • Signal Transduction / drug effects*
  • Staurosporine / analogs & derivatives
  • Time Factors
  • Transplantation, Heterologous
  • Tumor Cells, Cultured / drug effects

Substances

  • Alkaloids
  • Antineoplastic Agents
  • Estrogens
  • 7-hydroxystaurosporine
  • Doxorubicin
  • Cyclophosphamide
  • Protein Kinase C
  • Staurosporine
  • Fluorouracil