IL-10 enhances B-cell IgE synthesis by promoting differentiation into plasma cells, a process that is inhibited by CD27/CD70 interaction

Clin Exp Immunol. 2002 Sep;129(3):446-52. doi: 10.1046/j.1365-2249.2002.01932.x.


Interleukin-10 (IL-10) is a major regulatory cytokine of inflammatory responses that is considered to play an important role in specific immunotherapy. However, whether IL-10 enhances or inhibits B-cell IgE production has remained a matter of contention. To clarify the effect of IL-10 on IgE synthesis in the presence of IL-4 and CD40 signalling, we examined B-cell proliferation, germline epsilon transcripts and plasma cell differentiation. In addition, the effect of CD27 signalling on IgE synthesis in the presence of IL-10, IL-4 and CD40 signalling was investigated. IL-10 facilitated the production of IgE in mononuclear cells and highly purified B-cells, enhanced B-cell proliferation and, most importantly, promoted the generation of plasma cells. However, IL-10 did not enhance expression of germline epsilon transcripts. The addition of CD27 signalling through the use of CD32-CD27 ligand (CD70) double transfectants significantly diminished the B-cell proliferation, IgE synthesis and plasma cell differentiation enhanced by IL-10. IL-10 enhances B-cell IgE production by promoting differentiation into plasma cells. CD27/CD70 interactions under IL-10 and sufficient CD40 cosignalling exert the opposite effect on IgE synthesis. The results of this study indicate that precautions are critical when planning immunotherapy using IL-10 in IgE-related allergic diseases.

MeSH terms

  • Adult
  • Antigens, CD*
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • CD27 Ligand
  • Cell Differentiation
  • Cells, Cultured
  • Humans
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / genetics
  • Interleukin-10 / antagonists & inhibitors
  • Interleukin-10 / pharmacology*
  • Lymphocyte Activation / drug effects
  • Membrane Proteins / physiology*
  • Plasma Cells / immunology
  • RNA, Messenger / biosynthesis
  • Signal Transduction
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / physiology*


  • Antigens, CD
  • CD27 Ligand
  • CD70 protein, human
  • Membrane Proteins
  • RNA, Messenger
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Interleukin-10
  • Immunoglobulin E