[Nano-ESI-MS/MS identification on apoptosis qssociated proteins induced by inhibiting ubiquitin-proteasome pathway]

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Sep;34(5):630-4.
[Article in Chinese]

Abstract

CapLC-ESI-MS/MS and nano-ESI-MS/MS techniques were used to identify the apoptosis associated proteins induced by inhibiting the ubiquitin-proteasome pathway in Mo7e leukaemic cells. In 2-DE, spot H was found to initiate its overexpression at 2 h after the inhibition and reached its peak at 6 h. It was identified as Rho GDI beta protein after the tandem mass spectrum and after the sequence of its tryptic peptides were obtained by the ESI-MS/MS techniques. It was not revealed by peptide mass fingerprint using MALDI-TOF-MS. Other two spots induced by the inhibition appeared close to spot H were also revealed identical to Rho GDI brg;, possibly due to unknown modifications.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Cysteine Endopeptidases
  • Guanine Nucleotide Dissociation Inhibitors / analysis*
  • Multienzyme Complexes / antagonists & inhibitors*
  • Neoplasm Proteins / analysis*
  • Proteasome Endopeptidase Complex
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tumor Cells, Cultured
  • Ubiquitin / antagonists & inhibitors*
  • rho GTP-Binding Proteins / analysis*

Substances

  • Guanine Nucleotide Dissociation Inhibitors
  • Multienzyme Complexes
  • Neoplasm Proteins
  • Ubiquitin
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • rho GTP-Binding Proteins