Pax-2 interacts with RB and reverses its repression on the promoter of Rig-1, a Robo member

Biochem Biophys Res Commun. 2002 Aug 30;296(4):1019-25. doi: 10.1016/s0006-291x(02)02032-6.

Abstract

RB plays dual roles in the regulation of cell proliferation and differentiation. The nervous tissue-specific gene Rig-1, a member of the roundabout (Robo) guidance receptor family, was identified as an RB-regulated gene in the mouse embryo. Herein, we report that a 2.3kb genomic DNA fragment, which contains the first 129 bases of the 5'-untranslated region and 2.2kb of the 5'-flanking region of Rig-1, has a cell type-specific promoter activity. Rig-1 promoter activity is downregulated by RB and upregulated by Pax-2. Furthermore, Rig-1 and Pax-2 mRNAs are coexpressed in the hindbrain and spinal cord of the E11.5 mouse embryo, suggesting that Pax-2 may regulate Rig-1 expression during the embryonic stage. Pax-2 interacts with RB and reverses its transcriptional suppression on the Rig-1 promoter. In summary, the ubiquitous tumor suppressor RB and the neuron-enriched transcription factor Pax-2 may play a role in the regulation of Rig-1 expression during embryogenesis.

MeSH terms

  • 3T3 Cells
  • 5' Untranslated Regions
  • Animals
  • Brain / metabolism
  • DNA / metabolism
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins / metabolism*
  • Down-Regulation
  • Genes, Reporter
  • Immunoblotting
  • In Situ Hybridization
  • Luciferases / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism*
  • PAX2 Transcription Factor
  • Precipitin Tests
  • Promoter Regions, Genetic*
  • Protein Binding
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface
  • Receptors, Immunologic / genetics
  • Retinoblastoma Protein / metabolism*
  • Spinal Cord / metabolism
  • Time Factors
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transfection
  • Up-Regulation

Substances

  • 5' Untranslated Regions
  • DNA, Complementary
  • DNA-Binding Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • PAX2 Transcription Factor
  • Pax2 protein, mouse
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • Retinoblastoma Protein
  • Robo3 protein, mouse
  • Transcription Factors
  • roundabout protein
  • DNA
  • Luciferases

Associated data

  • GENBANK/AF532971