Molecular background of Rh D-positive, D-negative, D(el) and weak D phenotypes in Chinese

C-P Shao; J-H Maas; Y-Q Su; M Köhler; T J Legler

doi:10.1046/j.1423-0410.2002.00192.x

Molecular background of Rh D-positive, D-negative, D(el) and weak D phenotypes in Chinese

Vox Sang. 2002 Aug;83(2):156-61. doi: 10.1046/j.1423-0410.2002.00192.x.

Authors

C-P Shao¹, J-H Maas, Y-Q Su, M Köhler, T J Legler

Affiliation

¹ Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Strasse 40, 37075 Göttingen, Germany.

PMID: 12201845
DOI: 10.1046/j.1423-0410.2002.00192.x

Abstract

Background and objectives: The aim of this study was to elucidate the genetic background of D-negative and D(el) in the Chinese population.

Materials and methods: We investigated nine D-positive, 76 D-negative, 26 D(el) and three weak D Chinese individuals by amplification and sequencing of the complete coding region of the RHD gene from genomic DNA. A new RHD polymerase chain reaction with sequence-specific primers (PCR-SSP) method was developed with optimized specificity for typing Chinese individuals.

Results: In D-positive samples the RHD sequence was in complete concordance with RHD in other populations. In 12 of 76 (15.8%) D-negative individuals we detected regions of RHD. Three new alleles were found. All 26 D(el), as well as two weak D, individuals carried an RHD 1227A allele. In the remaining weak D sample we identified a weak D type 15.

Conclusions: It should now be possible to correctly predict the RhD phenotype in Chinese subjects. D(el) can also be designated as a particular weak D type.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Asian People / genetics
China / epidemiology
DNA Primers / standards
Gene Frequency
Genotype
Humans
Phenotype
Polymerase Chain Reaction
Rh-Hr Blood-Group System / genetics*
Sensitivity and Specificity

Substances

DNA Primers
Rh-Hr Blood-Group System