Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA

J Clin Microbiol. 2002 Sep;40(9):3485-8. doi: 10.1128/JCM.40.9.3485-3488.2002.

Abstract

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Burkholderia Infections / diagnosis
  • Burkholderia Infections / epidemiology
  • Burkholderia Infections / microbiology
  • Burkholderia cepacia / classification*
  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / isolation & purification*
  • Child
  • Child, Preschool
  • Cystic Fibrosis / epidemiology
  • Cystic Fibrosis / microbiology*
  • Czech Republic / epidemiology
  • DNA, Bacterial / analysis*
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Polymerase Chain Reaction / methods*
  • Rec A Recombinases / genetics
  • Sensitivity and Specificity
  • Sputum / chemistry*

Substances

  • DNA, Bacterial
  • Rec A Recombinases