Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings

Proteomics. 2002 Aug;2(8):947-59. doi: 10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.CO;2-J.


Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate peroxidase protein(s) was accompanied by rapid changes in the 2-DE protein profiles, over controls. Out of a total of 56 proteins investigated, which were reproducible in repeated experiments, 52 protein spots were visually identified as differentially expressed over controls. Six proteins were N-terminally blocked, and the sequence of 14 proteins could not be determined, whereas 36 proteins were N-terminally and one was internally sequenced. Ozone caused drastic reductions in the major leaf photosynthetic proteins, including the abundantly present ribulose-1, 5-bisphosphate carboxylase/oxygenase, and induction of various defense/stress related proteins. Most prominent change in leaves, within 24 h post-treatment with ozone, was the induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, ascorbate peroxidase(s), superoxide dismutase, calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein. Present results demonstrate the highly damaging effect of ozone on rice seedlings at the level of the proteome.

MeSH terms

  • Air Pollutants / toxicity
  • Amino Acid Sequence
  • Ascorbate Peroxidases
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation, Plant
  • Molecular Sequence Data
  • Oryza / drug effects
  • Oryza / physiology*
  • Oxidants, Photochemical / pharmacology*
  • Oxidants, Photochemical / toxicity
  • Oxidative Stress
  • Ozone / toxicity*
  • Peroxidases / metabolism
  • Plant Proteins / analysis*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Proteome*
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Seedlings / drug effects
  • Seedlings / physiology


  • Air Pollutants
  • Oxidants, Photochemical
  • Plant Proteins
  • Proteome
  • pathogenesis-related proteins, plant
  • Ozone
  • Peroxidases
  • Ascorbate Peroxidases
  • Ribulose-Bisphosphate Carboxylase