BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain

Mech Dev. 2002 Sep;117(1-2):123-35. doi: 10.1016/s0925-4773(02)00194-6.

Abstract

In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and--unusually among vertebrates--in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine (BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16 h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows--in conjunction with a Hu-assay demonstrating earliest neuronal differentiation--that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD--a marker for freshly determined neuronal cells--confirms this unusual subpial postembryonic forebrain neurogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Bromodeoxyuridine / metabolism*
  • Cell Differentiation
  • Cell Division
  • ELAV Proteins
  • Immunohistochemistry
  • In Situ Hybridization
  • Larva / cytology
  • Larva / growth & development
  • Larva / metabolism
  • Mitosis
  • Nerve Tissue Proteins / genetics*
  • Neurons / cytology
  • Neurons / metabolism
  • Proliferating Cell Nuclear Antigen / metabolism
  • Prosencephalon / cytology
  • Prosencephalon / growth & development*
  • Prosencephalon / metabolism*
  • RNA-Binding Proteins / metabolism*
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Zebrafish / genetics
  • Zebrafish / growth & development*
  • Zebrafish / metabolism*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • ELAV Proteins
  • Nerve Tissue Proteins
  • Proliferating Cell Nuclear Antigen
  • RNA-Binding Proteins
  • NeuroD protein
  • Bromodeoxyuridine