Ceftazidime-hydrolysing CTX-M-15 Extended-Spectrum Beta-Lactamase (ESBL) in Poland

J Antimicrob Chemother. 2002 Sep;50(3):393-6. doi: 10.1093/jac/dkf151.

Abstract

The CTX-M-15 extended-spectrum beta-lactamase (ESBL) was recently identified in Enterobacteriaceae isolates in India, and demonstrated significant hydrolytic activity against ceftazidime, in contrast to the majority of CTX-M enzymes. CTX-M-15 differs from CTX-M-3, which is one of the most prevalent ESBLs in Poland, by only a single amino acid change (Asp-240-->Gly). Three cefotaxime- and ceftazidime-resistant Enterobacteriaceae isolates, recovered during 1998-2000 in two Polish hospitals, were found to produce CTX-M-15. Similar to those from India, the isolates contained the ISEcp1 insertion sequence located upstream of the bla(CTX-M-15) gene, which has been recently demonstrated to mobilize 3'-adjacent genes to transfer between DNA replicons. However, its different position with respect to the beta-lactamase gene indicated the independent selection of the ESBL gene in the two countries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Ceftazidime / pharmacology*
  • Conjugation, Genetic
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Genes, Bacterial / genetics*
  • Hospitals, Urban
  • Humans
  • Molecular Sequence Data
  • Poland / epidemiology
  • Serratia marcescens / drug effects
  • Serratia marcescens / enzymology
  • Serratia marcescens / genetics
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Ceftazidime
  • beta-lactamase CTX-M-15
  • beta-Lactamases

Associated data

  • GENBANK/AJ310929