Basic fibroblast growth factor, glutamine synthetase, and interleukin-6 in vitreous fluid from eyes with retinal detachment complicated by proliferative vitreoretinopathy

Am J Ophthalmol. 2002 Sep;134(3):367-75. doi: 10.1016/s0002-9394(02)01536-2.

Abstract

Purpose: To investigate the presence of basic fibroblast growth factor (bFGF), glutamine synthetase (GS), and interleukin-6 (IL-6) in vitreous fluid from eyes with retinal detachment complicated by proliferative vitreoretinopathy (PVR).

Design: Comparative case series; experimental study.

Methods: In a prospective study, we measured bFGF, GS, IL-6, and total protein in vitreous fluid samples from 53 eyes from 53 consecutive patients with PVR operated on in our hospital. As controls, vitreous fluid samples from eyes with a macular hole (n = 9) or pucker (n = 11) were used.

Main outcome measures: Laboratory data of the patient group were compared with the control group and correlated with various clinical data, especially with visual recovery and redetachment.

Results: For IL-6, bFGF, and total protein we found significantly higher levels in PVR patients' eyes than in control eyes (P =.03, P =.046, and P <.0001, respectively). Within the PVR group, no significant correlation was found for IL-6, bFGF, GS, or total protein with the various tested clinical variables.

Conclusions: We found increased levels of IL-6, bFGF, and total protein in vitreous fluid from patients' eyes with PVR. Whether the increased levels of IL-6, bFGF, and total protein are the result of an injury-induced upregulation of these proteins as part of a self-protective mechanism of the retina to minimize photoreceptor damage after the mechanical injury induced by retinal detachment is, at present, not known.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Enzyme-Linked Immunosorbent Assay
  • Eye Proteins / metabolism
  • Female
  • Fibroblast Growth Factor 2 / metabolism*
  • Glutamate-Ammonia Ligase / metabolism*
  • Humans
  • Interleukin-6 / metabolism*
  • Male
  • Middle Aged
  • Prospective Studies
  • Retinal Detachment / etiology
  • Retinal Detachment / metabolism*
  • Retinal Detachment / surgery
  • Vitrectomy
  • Vitreoretinopathy, Proliferative / complications
  • Vitreoretinopathy, Proliferative / metabolism*
  • Vitreoretinopathy, Proliferative / surgery
  • Vitreous Body / metabolism*

Substances

  • Eye Proteins
  • Interleukin-6
  • Fibroblast Growth Factor 2
  • Glutamate-Ammonia Ligase