Orexin signaling in recombinant neuron-like cells

FEBS Lett. 2002 Aug 28;526(1-3):11-4. doi: 10.1016/s0014-5793(02)03101-0.


To assess the role of orexin receptor signaling in neuron-like cells, Neuro-2a murine neuroblastoma and PC12 human pheochromocytoma cells were stably transfected with human OX(1) or OX(2) receptors. Activation of both receptors strongly elevated cellular inositol phosphates and Ca(2+). A difference in the potency between orexin-A and -B was seen for OX(1), but not OX(2) receptors. Dependence of the orexin-mediated Ca(2+) response on extracellular Ca(2+) and the observed Ba(2+) influx indicate that in addition to phospholipase C, orexin receptors also may couple to similar non-voltage-gated Ca(2+) channels in neuronal cells as previously characterized in non-neuronal cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / physiology
  • Carrier Proteins / physiology*
  • Humans
  • Inositol Phosphates / metabolism
  • Intracellular Signaling Peptides and Proteins*
  • Neuroblastoma
  • Neurons
  • Neuropeptides / physiology*
  • Neurotransmitter Agents / physiology
  • Orexin Receptors
  • Orexins
  • PC12 Cells
  • Pheochromocytoma
  • Rats
  • Receptors, G-Protein-Coupled
  • Receptors, Neuropeptide / physiology*
  • Recombinant Proteins / metabolism
  • Transfection
  • Tumor Cells, Cultured


  • Carrier Proteins
  • HCRT protein, human
  • Inositol Phosphates
  • Intracellular Signaling Peptides and Proteins
  • Neuropeptides
  • Neurotransmitter Agents
  • Orexin Receptors
  • Orexins
  • Receptors, G-Protein-Coupled
  • Receptors, Neuropeptide
  • Recombinant Proteins
  • Calcium