Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids

J Virol. 2002 Oct;76(19):10069-73. doi: 10.1128/jvi.76.19.10069-10073.2002.

Abstract

The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid / metabolism*
  • Gene Products, pol / metabolism*
  • RNA, Viral / physiology*
  • Spumavirus / physiology*
  • Virion / physiology
  • Virus Assembly*

Substances

  • Gene Products, pol
  • RNA, Viral