Plasma glucose and insulin reduction after consumption of breads varying in amylose content

Eur J Clin Nutr. 2002 Sep;56(9):913-20. doi: 10.1038/sj.ejcn.1601411.


Objective: Consumption of a meal high in amylose starch (70%) decreases peak insulin and glucose levels and area under the curve (AUC). The objective was to determine the amount of amylose necessary in a meal for the beneficial decrease in glucose or insulin to occur.

Design: Twenty-five subjects, 13 men (averaging 88.1 kg, 41 y, and 27.9 body mass index) and 12 women (averaging 72.4 kg, 41 y and 27.1 body mass index) were given six tolerance tests in a Latin Square design: glucose alone (1 g glucose/kg body weight) and five breads (1 g carbohydrate/kg body weight) made with 70% amylose cornstarch, standard cornstarch (30% amylose), and blends of the two starches (40, 50 and 60% amylose starch). A standard menu was fed for 3 days. One subject withdrew from the study.

Results: Glucose, insulin and glucagon response to the carbohydrate loads was similar in men and women. Peak glucose response was lowest after the breads containing 50-70% amylose starch. AUC was significantly higher after the glucose load than after all bread loads. The lowest AUCs occurred after the 60 and 70% amylose starch breads. Insulin response and AUC were significantly lower after the 60 and 70% amylose starch breads than after the glucose or the other breads.

Conclusion: Results indicate that the amylose content of the starch used in the acute meal needs to be greater than 50% to significantly reduce plasma glucose and insulin in men and women.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Amylose / pharmacology*
  • Blood Glucose / metabolism*
  • Bread*
  • Dietary Carbohydrates / pharmacology*
  • Fasting
  • Female
  • Humans
  • Insulin / blood*
  • Male
  • Middle Aged
  • Reference Values


  • Blood Glucose
  • Dietary Carbohydrates
  • Insulin
  • Amylose