Probing cell surface charge by scanning electrode potential

Electrophoresis. 2002 Jul;23(13):2080-6. doi: 10.1002/1522-2683(200207)23:13<2080::AID-ELPS2080>3.0.CO;2-W.

Abstract

A static mercury electrode was used for measurement of double-layer charge displacement signals caused by living plant cells of the unicellular marine alga Dunaliella tertiolecta. By scanning the electrode potential a point is reached where the charge density of a plant cell compensates the electrode charge density. The experimentally determined values of surface charges for unicellular marine alga Dunaliella tertiolecta cells are -0.63 and -0.75 nuC/cm(2) in 0.1 M NaCl and 1 M NaCl solutions, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / physiology*
  • Cell Membrane / ultrastructure
  • Chlorophyta / cytology
  • Chlorophyta / physiology*
  • Electrochemistry / methods
  • Electrophoresis / methods
  • Electrophysiology / methods
  • Microelectrodes