Genetic interactions link ARF1, YPT31/32 and TRS130

Yeast. 2002 Sep 15;19(12):1075-86. doi: 10.1002/yea.903.

Abstract

A genetic screen for synthetic lethal interactions with arf1(-) identified a recessive mutation in TRS130, one of 10 components in the trafficking protein particle (TRAPP) complex (Sacher et al., 2000). As TRS130 is an essential gene, the synthetic lethal allele (trs130-101) is a novel one that requires ARF1 for viability. This allele was found to exhibit no defects in secretory function, i.e. processing of carboxypeptidase Y or invertase. YPT31 and YPT32 were identified in a subsequent screen as high-copy suppressors of arf1(-)trs130-101. Increasing the gene dosage of YPT31/32 also suppressed lethality resulting from deletion of TRS130 or TRS120 but not three other essential TRAPP subunit-encoding genes. Although unable to suppress defects in several alleles of ARF1, increasing the gene dosage of YPT31/32 suppressed the cold sensitivity of gcs1(-), an Arf GTPase-activating protein (GAP). Thus, these genetic interactions provide initial evidence for linkage of Arf and TRAPP signalling and for Ypt31/32 proteins functioning downstream of both components in the TRAPP complex and of Arf signalling via the Gcs1 Arf GAP.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ADP-Ribosylation Factor 1 / genetics*
  • ADP-Ribosylation Factor 1 / metabolism
  • Alleles
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • GTPase-Activating Proteins*
  • Gene Dosage
  • Gene Expression Regulation, Fungal
  • Genes, Suppressor
  • Glycoside Hydrolases / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mutation
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction
  • Vesicular Transport Proteins*
  • Yeasts / genetics*
  • Yeasts / growth & development
  • Yeasts / metabolism
  • beta-Fructofuranosidase
  • rab GTP-Binding Proteins / genetics*
  • rab GTP-Binding Proteins / metabolism

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • GCS1 protein, S cerevisiae
  • GTPase-Activating Proteins
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins
  • transport protein particle, TRAPP
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • YPT31 protein, S cerevisiae
  • YPT32 protein, S cerevisiae
  • ADP-Ribosylation Factor 1
  • rab GTP-Binding Proteins