Disruption of segmental neural crest migration and ephrin expression in delta-1 null mice

Dev Biol. 2002 Sep 1;249(1):121-30. doi: 10.1006/dbio.2002.0756.

Abstract

Neural crest cells migrate segmentally through the rostral half of each trunk somite due to inhibitory influences of ephrins and other molecules present in the caudal-half of somites. To examine the potential role of Notch/Delta signaling in establishing the segmental distribution of ephrins, we examined neural crest migration and ephrin expression in Delta-1 mutant mice. Using Sox-10 as a marker, we noted that neural crest cells moved through both rostral and caudal halves of the somites in mutants, consistent with the finding that ephrinB2 levels are significantly reduced in the caudal-half somites. Later, mutant embryos had aberrantly fused and/or reduced dorsal root and sympathetic ganglia, with a marked diminution in peripheral glia. These results show that Delta-1 is essential for proper migration and differentiation of neural crest cells. Interestingly, absence of Delta-1 leads to diminution of both neurons and glia in peripheral ganglia, suggesting a general depletion of the ganglion precursor pool in mutant mice.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Movement / genetics*
  • DNA-Binding Proteins / metabolism
  • Ephrin-B2
  • Ganglia, Spinal / embryology
  • Ganglia, Spinal / metabolism
  • Gene Expression Regulation, Developmental
  • High Mobility Group Proteins / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Mutant Strains
  • Neural Crest / cytology
  • Neural Crest / embryology*
  • Neural Crest / metabolism*
  • Neuroglia / metabolism
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Receptor, EphB2
  • Receptors, Notch
  • SOXE Transcription Factors
  • Transcription Factors

Substances

  • DNA-Binding Proteins
  • Ephrin-B2
  • High Mobility Group Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Receptors, Notch
  • SOXE Transcription Factors
  • Sox10 protein, mouse
  • Transcription Factors
  • delta protein
  • Receptor Protein-Tyrosine Kinases
  • Receptor, EphB2