[Reliable micromethod for determination of the protein content in tissue homogenates]

Acta Biol Med Ger. 1975;34(9):1441-6.
[Article in German]


A micromodification for protein determination in tissue material using Amido black 10 B is described. Compared with the method of LOWRY et al. it requires a comparable time expenditure, but has three principal advantages: 1) it is 5-10fold more sensitive; 2) the calibration curve is linear over a virtually unlimited range; 3) it is feasible in the presence of a number of substances frequently used in protein analyses and making difficult or impossible measurement according to LOWRY et al.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Brain / metabolism
  • Buffers
  • Cerebral Cortex / metabolism
  • Hippocampus / metabolism
  • Indicators and Reagents
  • Kidney / metabolism
  • Liver / metabolism
  • Micromanipulation
  • Proteins / analysis
  • Proteins / metabolism*
  • Rats
  • Visual Cortex / metabolism


  • Buffers
  • Indicators and Reagents
  • Proteins