Transforming growth factor-beta1-modulated cerebral gene expression

J Cereb Blood Flow Metab. 2002 Sep;22(9):1114-23. doi: 10.1097/00004647-200209000-00009.


Transforming growth factor-beta1 (TGF-beta1) plays a central role in the response of the brain to different types of injury. Increased TGF-beta1 has been found in the central nervous system of patients with acute or chronic disorders such as stroke or Alzheimer disease. To further define the molecular targets of TGF-beta1 in cerebral tissues, a selection of high-density cDNA arrays was used to characterize the mRNA expression profile of 7,000 genes in transgenic mice overexpressing TGF-beta1 from astrocytes as compared with the wild-type line. Selected findings were further evaluated by reverse transcription-polymerase chain reactions from independent transgenic and wild-type mice. Furthermore, the expression pattern of seven selected genes such as Delta-1, CRADD, PRSC-1, PAI-1, Apo-1/Fas, CTS-B, and TbetaR-II were confirmed in either cultured cortical neurons or astrocytes following TGF-beta1 treatment. The authors' observations enlarge the repertoire of known TGF-beta1-modulated genes and their possible involvement in neurodegenerative processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Astrocytes / metabolism*
  • Base Sequence
  • Cells, Cultured
  • Cerebral Cortex / metabolism*
  • DNA Primers
  • Gene Expression Regulation / physiology*
  • Glial Fibrillary Acidic Protein / genetics
  • Humans
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins / genetics*
  • Neurons / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Transcription, Genetic
  • Transforming Growth Factor beta / physiology*
  • Transforming Growth Factor beta1


  • DNA Primers
  • Glial Fibrillary Acidic Protein
  • Nerve Tissue Proteins
  • RNA, Messenger
  • TGFB1 protein, human
  • Tgfb1 protein, mouse
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1