Abstract
Megakaryocytes, the platelet precursors, are induced to differentiate in response to Mpl ligand. Here we report that stability of the megakaryocyte-specific platelet factor 4 (PF4) mRNA is substantially augmented in the presence of Mpl ligand. This stabilization requires protein synthesis, but the 3'-untranslated region of PF4 mRNA is not sufficient for granting the effect. This cytokine also significantly or mildly stabilizes Mpl receptor or GAPDH mRNAs, respectively, in contrast to a previously reported lack of effect on P2Y(1) receptor mRNA. Our study is the first to suggest that Mpl ligand-induced lineage specification is also determined by message stabilization.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
3' Flanking Region
-
3' Untranslated Regions
-
Animals
-
Cell Differentiation / drug effects
-
Cells, Cultured
-
Glyceraldehyde-3-Phosphate Dehydrogenases / drug effects
-
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
-
Megakaryocytes / cytology*
-
Megakaryocytes / drug effects
-
Mice
-
Neoplasm Proteins / genetics
-
Platelet Factor 4 / drug effects
-
Platelet Factor 4 / genetics*
-
Promoter Regions, Genetic
-
Proto-Oncogene Proteins / genetics
-
RNA Stability*
-
RNA, Messenger / drug effects*
-
Receptors, Cytokine / genetics
-
Receptors, Purinergic P2 / drug effects
-
Receptors, Purinergic P2 / genetics
-
Receptors, Purinergic P2Y1
-
Receptors, Thrombopoietin
-
Thrombopoietin / pharmacology*
Substances
-
3' Untranslated Regions
-
Neoplasm Proteins
-
P2RY1 protein, human
-
P2ry1 protein, mouse
-
Proto-Oncogene Proteins
-
RNA, Messenger
-
Receptors, Cytokine
-
Receptors, Purinergic P2
-
Receptors, Purinergic P2Y1
-
Receptors, Thrombopoietin
-
MPL protein, human
-
Platelet Factor 4
-
Thrombopoietin
-
Glyceraldehyde-3-Phosphate Dehydrogenases