[Polymorphism in codon 72 of the p53 gene and cervico-uterine cancer risk in Mexico]

Ginecol Obstet Mex. 2002 Jul;70:344-8.
[Article in Spanish]

Abstract

A polymorphism at codon 72 in the p53 gen has been reported as a potential risk factor to cervical cancer (CC) because human papillomavirus (HPV) is more effective at degrading p53 Arg-72 than p53 Pro-72, making individuals homozygous for p53 Arg-72 seven times more likely to develop HPV-associated CC. As In Mexico the CC is a health public problem, we designed this study to determinate whether the p53 codon 72 polymorphism represent a risk factor to CC in our population. A case-controls study was performed. DNA was obtained from paraffin-embedded cervical fixed tissue samples. Analysis of the p53 genotype at position 72 was performed by polymerase chain reaction using specific primers and Accll digestion. Among cases with CC the proportions of the p53 genotypes at codon 72 were 0.05 to proline homozygous, 0.5 to heterozygous, and 0.45 to arginine-homozygous. In controls the proportions were 0.08, 0.62, and 0.31. X2 test showed no significant difference In the proportions. We conclude than In our population, as other worldwide countries, the homozygous for arginine at codon 72 of the p53 gene is not a risk factor to cervical cancer.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Arginine / chemistry
  • Case-Control Studies
  • Codon / genetics*
  • DNA Mutational Analysis
  • DNA-Binding Proteins*
  • Exons / genetics
  • Female
  • Gene Frequency
  • Genes, p53*
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Mexico / epidemiology
  • Oncogene Proteins, Viral / metabolism
  • Papillomaviridae / metabolism
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Proline / chemistry
  • Repressor Proteins*
  • Risk Factors
  • Substrate Specificity
  • Tumor Suppressor Protein p53 / chemistry
  • Tumor Suppressor Protein p53 / metabolism
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / virology

Substances

  • Codon
  • DNA-Binding Proteins
  • E6 protein, Human papillomavirus type 16
  • E6 protein, Human papillomavirus type 18
  • Oncogene Proteins, Viral
  • Repressor Proteins
  • Tumor Suppressor Protein p53
  • Arginine
  • Proline