The root of Isatis indigotica (Cruciferae), popularly known as Ban-Lan-Gen is used in traditional Chinese medicine for seasonal febrile diseases, pestilence, mumps, eruptive diseases, inflammatory diseases with redness of skin, sore throat, etc. In this study, we evaluated the antinociceptive, antiinflammatory and antipyretic effects of Isatis indigotica root methanolic extract. The results showed that Isatis indigotica root extract significantly and dose-dependently inhibited the writhing responses of mice and decreased the licking time in both the early and late phases of the formalin test. It also reduced the paw edema induced by carrageenan in rats. In addition, it potently attenuated pyrexia induced by lipopolysaccharide.