[Fungal contamination detection in cornea preservation media]

J Fr Ophtalmol. 2002 Jun;25(6):590-3.
[Article in French]

Abstract

Purpose: Fungi can cause major complications following corneal grafting. In this study we aimed to verify the efficiency of a fungal contamination detection protocol for human corneas in preservation before clinical use.

Materials and methods: The 12 most frequently found species of fungi responsible for keratitis and endophthalmitis were inoculated to preservation medium used by most French eye banks. A protocol used by several centers, including a daily visual control, was followed in order to check that it showed the presence of all microorganisms, particularly slow-growing filamentous fungi.

Results: Every species was detected in a time from 2 to 4 days.

Conclusion: This microbiological contamination detection protocol of detection for human corneas in organ culture at +31 degrees C, seems to effectively detect the main agents responsible for fungal contamination.

MeSH terms

  • Candida / classification
  • Candida / isolation & purification*
  • Cornea / microbiology*
  • Corneal Transplantation
  • Fungi / classification
  • Fungi / isolation & purification*
  • Humans
  • Organ Preservation / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spores, Fungal / isolation & purification